NEWS

In February 2019, Dr. Shen began directing the Analytical Redox Biology Core within the COBRE program (P20GM121307, PI: Dr. Chris Kevil).

The Shen Lab combines mass spectrometry-based proteomics, redox biology, and machine learning to explore new diagnostic biomarkers and identify therapeutic targets in diseases such as cancer, diabetes, cardiovascular disease, and neurodegenerative disorders.

CURRENT PROJECTS

1. Proteomics applications in pancreatic cancer. Pancreatic cancer is known for its aggressive nature and poor prognosis. This project focuses on screening for early circulating biomarkers of pancreatic cancer and identifying potential therapeutic strategies.
2. Redox regulation of hyperglycemia in human disease. Hyperglycemia is a major risk factor for endothelial dysfunction and vascular complications. This project investigates the change in high glucose-induced oxidative stress and the corresponding redox regulation, providing potential therapeutic strategies to mitigate redox stress in conditions such as diabetes and Alzheimer's disease.
3. Machine learning and deep learning in proteomics. Proteomics data is ideally suited for Machine Learning. This project utilizes advanced machine learning and deep learning techniques to analyze proteomics datasets, with a focus on integrating and extracting insights from basic research, clinical research, and bioinformatics.

MAIN EQUIPMENT

1. ThermoFisher Orbitrap Exploris 480 coupled with the UltiMate™ NCS-3500RS nano-HPLC system
2. The SCIEX 7500 QTRAP® system, activated for QTRAP functionality and integrated with the Shimadzu LC-40 UHPLC
3. Shimadzu Prominence HPLC (LC 20A) with a fluorescence detector (RF-20 Axs), UV-vis detector (SPD-20AV), and fraction collector
4. Agilent system that includes 8890 GC and 8335 sulfur chemiluminescence detector
5. Nitric oxide Analyzer (nCLD 88, Eco Physics)

Selected Publications

1. Watts M, Kolluru GK, Dherange P, Pardue S, Si M, Shen X, Trosclair K, Glawe J, Al-Yafeai Z, Iqbal M, Pearson BH, Hamilton KA, Orr AW, Glasscock E, Kevil CG, Dominic P. Decreased bioavailability of hydrogen sulfide links vascular endothelium and atrial remodeling in atrial fibrillation. Redox Biol. 2021 Jan;38:101817. doi: 10.1016/j.redox.2020.101817.
2. Herrera GA, Del Pozo-Yauner L, Teng J, Zeng C, Shen X, Moriyama T, Ramirez Alcantara V, Liu B, Turbat-Herrera EA. Glomerulopathic Light Chain-Mesangial Cell Interactions: Sortilin-Related Receptor (SORL1) and Signaling. Kidney Int Rep. 2021 Mar 13;6(5):1379-1396.
3. Marutani E, Morita M, Hirai S, Kai S, Grange RMH, Miyazaki Y, Nagashima F, Traeger L, Magliocca A, Ida T, Matsunaga T, Flicker DR, Corman B, Mori N, Yamazaki Y, Batten A, Li R, Tanaka T, Ikeda T, Nakagawa A, Atochin DN, Ihara H, Olenchock BA, Shen X, Nishida M, Hanaoka K, Kevil CG, Xian M, Bloch DB, Akaike T, Hindle AG, Motohashi H, Ichinose F. Sulfide catabolism ameliorates hypoxic brain injury. Nat Commun. 2021 May 25;12(1):3108. doi: 10.1038/s41467-021-23363-x.
4. Takata T, Jung M, Matsunaga T, Ida T, Morita M, Motohashi H, Shen X, Kevil CG, Fukuto JM, Akaike T. Methods in sulfide and persulfide research. Nitric Oxide. 2021 Nov 1;116:47-64
5. Islam MZ, Shen X, Pardue S, Kevil CG, Shackelford RE. The ataxia-telangiectasia mutated gene product regulates the cellular acid-labile sulfide fraction. DNA Repair (Amst). 2022 Aug;116:103344. doi: 10.1016/j.dnarep.2022.103344.
6. Kolluru GK, Shackelford RE, Shen X, Dominic P, Kevil CG. Sulfide regulation of cardiovascular function in health and disease. Nat Rev Cardiol. 2023 Feb;20(2):109-125. doi: 10.1038/s41569-022-00741-6. Epub 2022 Aug 5. PMID: 35931887; PMCID: PMC9362470.
7. Dhaibar HA, Kamberov L, Carroll NG, Amatya S, Cosic D, Gomez-Torres O, Vital S, Sivandzade F, Bhalerao A, Mancuso S, Shen X, Nam H, Orr AW, Dudenbostel T, Bailey SR, Kevil CG, Cucullo L, Cruz-Topete D. Exposure to Stress Alters Cardiac Gene Expression and Exacerbates Myocardial Ischemic Injury in the Female Murine Heart. Int J Mol Sci. 2023 Jul 1;24(13):10994. doi: 10.3390/ijms241310994. PMID: 37446174; PMCID: PMC10341935.
8. Alam S, Pardue S, Shen X, Glawe JD, Yagi T, Bhuiyan MAN, Patel RP, Dominic PS, Virk CS, Bhuiyan MS, Orr AW, Petit C, Kolluru GK, Kevil CG. Hypoxia increases persulfide and polysulfide formation by AMP kinase dependent cystathionine gamma lyase phosphorylation. Redox Biol . 2023 Dec:68:102949. doi: 10.1016/j.redox.2023.102949. Epub 2023 Oct 30.
9. Pandey N, Kaur H, Chorawala MR, Anand SK, Chandaluri L, Butler ME, Aishwarya R, Gaddam SJ, Shen X, Alfaidi M, Wang J, Zhang X, Beedupalli K, Bhuiyan MS, Bhuiyan MAN, Buchhanolla P, Rai P, Shah R, Chokhawala H, Jordan JD, Magdy T, Orr AW, Stokes KY, Rom O, Dhanesha N. Interactions between integrin α9β1 and VCAM-1 promote neutrophil hyperactivation and mediate poststroke DVT. Blood Adv . 2024 May 14;8(9):2104-2117.
10. Chu X, Xu J, Shen X, Sheng W, Sun J, Gu Y, Lewis DF, Cooper D, Zoorob D, Wang Y. Altered Production and Cellular Levels of Hydrogen Sulfide (H₂S) in Placental Trophoblasts from Pregnancies Affected by Pre-Eclampsia. Pathophysiology. 2025 Mar 4;32(1):10. doi: 10.3390/pathophysiology32010010. PMID: 40137467; PMCID: PMC11946082

Complete List of Published Work in My Bibliography | Google Scholar

ALUMNI

Contact the Shen Lab

LSU Health Shreveport
Department of Pathology
1501 Kings Hwy
Shreveport, LA 71103

(318) 675-3371 office
(318) 675-5249 lab
(318) 675-8144 fax
E-mail: xinggui.shen@lsuhs.edu